A loop full of bacterial strain was inoculated in 30 ml of Nutrient broth in a conical flask and incubated for 72 hrs to get active strain by using agar well diffusion method. Muller Hinton Agar ...

Cube 1 has a surface area to volume ratio of 10 : 3 and has a rate of diffusion of .000259 cm/sec. However, Cube 2 and 3 have a ratio of 48.3 to 20.825 and 46.9 to 19.6 (respectively) and a rate of diffusion of .000276 cm/sec. Cube 2 and 3's ratios are smaller than Cube 1's but their rate of diffusion is faster than that of Cube 1's.

A cube of agar with a 1 cm side length, a cube of agar with a 1.5 cm side length, and a cube of agar with a 2cm side length were all cut out from a 2% agar sample containing NaOH and phenolphthalein using a serrated knife and a ruler. The surface areas and volumes of each cube were recorded in Table 1 using the metric ruler.

Prepare agar block using set amount of water, agar and phenolphthalein indicator. 2. Cut 5 agar cubes of lengths 0.2 cm, 0.4 cm, 0.6 cm, 0.8 cm and 1.0 cm carefully using a scalpel and a ruler for measurement. 3. Measure 25cm3 of hydrochloric acid using a measuring cylinder and empty it gently into each of the 5 beakers. 4.

1. Distance of Diffusion of vinegar (cm) = measure the clear part of the agar cube on only one side. Go to page 15 or use the measuring tool in lesson 3.09. 2. Distance of Diffusion in millimeters. = (Distance in centimeters) x 10 3. Rate of Diffusion (mm/minute) = Distance of Diffusion (mm) ÷ 5 minutes Page 18 R E S U LT S A N D C A L C U L ...

Factors affecting the rate of diffusion can be investigated using agar cubes. The agar cubes contain an indicator called phenolphthalein. Phenolphthalein is colourless, but turns pink in the presence of an alkali, such as sodium hydroxide solution. Cubes of agar, with sides 3 cm, 2 cm and 1 cm, are placed into sodium hydroxide solution for 10 ...

Osmosis and diffusion are in some ways alike. This experiment will be covering diffusion. The sodium hydroxide will mix with the phenolphthalein agar cubes and diffuse into the cubes. You will be able to see how much diffusion had occurred and compare the ratios of surface areas to volumes.

In this experiment, you will use agar cubes to which the indicator phenolphthalein has been added. Phenolphthalein is an acid/base indicator that turns pink in the presence of a base such as NaOH. Thus the surface of the agar cubes will turn pink immediately when put into a NaOH solution.

Agar Cube Diffusion. We use coloured agar cubes to see how different shapes change the rate of diffusion. Aerobic Respiration. We use maggots to let us observe aerobic respiration. Anaerobic Respiration. We use yeast to let us observe anaerobic respiration. Microbes in Milk.